BIOMOLECULES AND ENZYMES

Detailed explanation-1: -Mucic acid test is a test that is highly specific and is used for the detection of the presence of galactose and lactose. It is also termed galactaric acid that is named after the product of the reaction.

Detailed explanation-2: -Principle of Mucic acid test Monosaccharides, such as galactose contain sugars, such as lactose has a positive response to this chemical reaction. In the presence of acidic substances such as nitric acid create saccharic acids. The saccharic acid produced is insoluble and forms clear crystals.

Detailed explanation-3: -Crystals will be produced below the tube if the sample contains galactose or its derivatives. This is regarded as a positive result for mucic acid test.

Detailed explanation-4: -Reagent-Mucic acid reagent: concentrated nitric acid. Mucic acid Test Procedure-Mix 3 drops of the carbohydrate solution (galactose and lactose) and 3 drops of the concentrated Nitric Acid on a glass slide-Pass the mixture over a small flame until it is almost dry.