BIOLOGY
ENZYMES
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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they recreate the conditions from the bacteria at which the enzyme works
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they cut the DNA
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they allow the DNA to move through the gel
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they aren’t needed-it’s just a waste of time
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Detailed explanation-1: -The optimum temperature for the activity of most restriction enzymes is 37°C. Some enzymes, however, work best at 55°C, 65°C, or even 75°C!
Detailed explanation-2: -Major function of the buffer is to maintain pH of the reaction (usually, 8.0) and provide a favorable environment for the enzyme to function.
Detailed explanation-3: -Restriction Enzyme Classification. Restriction endonucleases are categorized into one of four general groups (Types I, II, III, and homing endonucleases based on their subunit structure, cofactor requirements, specificity of cleavage, and associated methylase activity (Table 1.2).
Detailed explanation-4: -Definition. A restriction enzyme is a protein isolated from bacteria that cleaves DNA sequences at sequence-specific sites, producing DNA fragments with a known sequence at each end. The use of restriction enzymes is critical to certain laboratory methods, including recombinant DNA technology and genetic engineering.