BIOLOGY
ENZYMES
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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use Benedict’s solution to measure the rate at which starch is used up
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use iodine to measure the rate at which glucose is produced
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use iodine to measure the rate at which starch is used up
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use Biuret’s solution to measure the rate at which glucose is produced
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Detailed explanation-1: -We can calculate the rate of the reaction by dividing the number one by the time taken in seconds. The calculation is therefore 1/t. Plot a graph of rate of reaction against pH. A similar experiment can be carried out to investigate the effect of temperature on amylase activity.
Detailed explanation-2: -Amylases digest starch into smaller molecules, ultimately yielding maltose, which in turn is cleaved into two glucose molecules by maltase.
Detailed explanation-3: -Measure the time taken for amylase to completely break down starch, by withdrawing samples at 10 second intervals and noting the time at which the solution no longer gives a blue-black colour with iodine solution (but the iodine solution remains orange). Use buffers to provide solutions at different pHs.
Detailed explanation-4: -decomposition reaction. Decomposition reactions are characterized by a larger molecule (e.g. starch) being broken down into smaller molecules (e.g. glucose) as a catabolic reaction.