BIOMOLECULES AND ENZYMES

Detailed explanation-1: -When DCPIP is added into lemon juice, the vitamin C reduces the dye, then, decolorizes the dye. Therefore, the decolorization of DCPIP indicates the presence of vitamin C in lemon juice.

Detailed explanation-2: -DCPIP is blue when dissolved in water and is red in acid conditions, and is reduced by ascorbic acid (vitamin C) to a colourless compound. However, in this titration, the end point colour change will be from blue to pink due to the presence of the phosphoric(V) acid used to prepare the vegetable extract.

Detailed explanation-3: -2, 6-dichlorophenolindophenol, ( DCPIP ) is a blue chemical compound used as a redox dye. Oxidized DCPIP is blue, reduced DCPIP is colorless.

Detailed explanation-4: -You can check the end point colour by testing a small amount of vitamin C with the DCPIP and observe the disappearance of the blue colour. The end point is usually a pink colour that persists for about 15 seconds or longer.

Detailed explanation-5: -DCPIP can also be used as an indicator for vitamin C. If vitamin C, which is a good reducing agent, is present, the blue dye, which turns pink in acid conditions, is reduced to a colorless compound by ascorbic acid.