BIOLOGY
MECHANISM OF ENZYME ACTION
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Emulsion test
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Biuret test
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Benedict’s test
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Iodine test
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Detailed explanation-1: -What is the Biuret Test? The biuret test is a chemical test that can be used to check for the presence of peptide bonds in a given analyte. Therefore, the biuret test can also be used to gauge the amount of protein present in the analyte.
Detailed explanation-2: -The test is named so because it also gives a positive reaction to the peptide-like bonds in the biuret molecule.
Detailed explanation-3: -The presence of protein is tested by the Biuret test for proteins. The Biurette reagent made of sodium hydroxide and copper (II) sulphate helps in determining the presence of protein in a sample.
Detailed explanation-4: -The biuret method, which uses the interaction of peptide bonds in proteins with cupric ions at alkaline pH to generate a coloured product that absorbs at 540–560 nm, is often used to assess total protein in plasma or serum.
Detailed explanation-5: -Proteins are detected using Biuret reagent . This turns a mauve or purple colour when mixed with protein.