BIOLOGY
NUCLEIC ACIDS
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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How effectively the helicase works
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What direction the nucleotides are pointing
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Where the RNA primer attaches
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Okazaki’s famous experiment
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Detailed explanation-1: -DNA is always synthesized in the 5’-to-3’ direction, meaning that nucleotides are added only to the 3’ end of the growing strand.
Detailed explanation-2: -DNA is always synthesized in the 5’ to 3’ direction because DNA polymerases can only catalyze phosphodiester bond formation (a condensation reaction) between the 3’ OH of the sugar of the last nucleotide of an existing strand and the 5’ phosphate group of the incoming nucleotide.
Detailed explanation-3: -DNA replication goes in the 5’ to 3’ direction because DNA polymerase acts on the 3’-OH of the existing strand for adding free nucleotides.
Detailed explanation-4: -RNA polymerase synthesizes an RNA strand complementary to a template DNA strand. It synthesizes the RNA strand in the 5’ to 3’ direction, while reading the template DNA strand in the 3’ to 5’ direction. The template DNA strand and RNA strand are antiparallel.