BIOMOLECULES AND ENZYMES

BIOLOGY

NUCLEIC ACIDS

Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
In which direction does the RNA polymerase move along the template strand of the DNA?
A
from 3’ to 5’
B
from 5’ to 3’
C
It can move in both directions
D
It depends on the strands
Explanation: 

Detailed explanation-1: -RNA polymerase synthesizes an RNA transcript complementary to the DNA template strand in the 5’ to 3’ direction. It moves forward along the template strand in the 3’ to 5’ direction, opening the DNA double helix as it goes.

Detailed explanation-2: -Once RNA polymerase and its related transcription factors are in place, the single-stranded DNA is exposed and ready for transcription. At this point, RNA polymerase begins moving down the DNA template strand in the 3’ to 5’ direction, and as it does so, it strings together complementary nucleotides.

Detailed explanation-3: -All mRNAs are read in the 5´ to 3´ direction, and polypeptide chains are synthesized from the amino to the carboxy terminus. Each amino acid is specified by three bases (a codon) in the mRNA, according to a nearly universal genetic code.

Detailed explanation-4: -DNA polymerases can only make DNA in the 5’ to 3’ direction, and this poses a problem during replication. A DNA double helix is always anti-parallel; in other words, one strand runs in the 5’ to 3’ direction, while the other runs in the 3’ to 5’ direction.

Detailed explanation-5: -The template strand is directed in the 5’ to 3’ direction. The coding strand has a complementary nucleotide sequence. The template strand does not have any complementary sequence.

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