BIOMOLECULES AND ENZYMES

Detailed explanation-1: -DNA ligase seals the gap between the molecules, forming a single piece of DNA.

Detailed explanation-2: -DNA polymerase I removes the RNA primer and fills in the gaps with DNA. However, DNA polymerase I cannot catalyze the reaction to remove the nicks. Another enzyme, DNA ligase, seals the nicks by forming the phosphodiester bond, thus generating a continuous sugar-phosphate backbone for the lagging strand.

Detailed explanation-3: -DNA Ligase enzymes seal the breaks in the backbone of DNA that are caused during DNA replication, DNA damage, or during the DNA repair process. The biochemical activity of DNA ligases results in the sealing of breaks between 5′-phosphate and 3′-hydroxyl termini within a strand of DNA.

Detailed explanation-4: -DNA ligase: An enzyme that seals gaps between Okazaki fragments on the lagging strand.