BIOMOLECULES AND ENZYMES

Detailed explanation-1: -What are Okazaki Fragments For? Okazaki fragments are short sections of DNA formed at the time of discontinuous synthesis of the lagging strand during replication of DNA. It is essential as it allows for the synthesis of both the daughter strands required for cell division.

Detailed explanation-2: -On the lagging strand, DNA synthesis restarts many times as the helix unwinds, resulting in many short fragments called “Okazaki fragments.” DNA ligase joins the Okazaki fragments together into a single DNA molecule.

Detailed explanation-3: -Lagging-strand replication is discontinuous, with short Okazaki fragments being formed and later linked together.

Detailed explanation-4: -Discontinuous DNA synthesis occurs from the 5’ end to the 3’ end of the parent strand. This strand is often referred to as the lagging strand. It is completed in short sequences of nucleotides called Okazaki fragments. Replication on the lagging strand begins with the addition of an RNA primer by the enzyme primase.

Detailed explanation-5: -This discontinuous synthesis results in the generation of fragments on the lagging strand called Okazaki fragments. DNA polymerase I recognizes a “nick” or break in the phosphate backbone, and then removes each RNA primer and fills the gaps with DNA. DNA ligase then covalently links the phosphate backbone.