BIOMOLECULES AND ENZYMES

Detailed explanation-1: -When a DNA solution is heated enough, the double-stranded DNA unwinds and the hydrogen bonds that hold the two strands together weaken and finally break. The process of breaking double-stranded DNA into single strands is known as DNA denaturation, or DNA denaturing.

Detailed explanation-2: -DNA denaturation is a process of separating dsDNA into single strands, which are favorable to DNA hybridization. Even though the denaturation is a key reaction that determines the success of DNA hybridization based bioassays, no systematic characterization of denaturation method for dsDNA has been attempted thus far.

Detailed explanation-3: -(C) The denaturation process is the reverse process of the hybridization reaction. At a given temperature, the equilibrium constant is determined by the change in free energy of the reaction (G0), which is strictly determined by the thermodynamic properties of the double-stranded and single-stranded DNA molecules.

Detailed explanation-4: -The separation of two DNA stands by heating is called denaturation. The intermolecular hydrogen bonds and disulfde linkages break.