BIOMOLECULES AND ENZYMES

Detailed explanation-1: -The process of breaking double-stranded DNA into single strands is known as DNA denaturation, or DNA denaturing. The temperature at which the DNA strands are half denatured, meaning half double-stranded, half single-stranded, is called the melting temperature(Tm).

Detailed explanation-2: -The separation of the two single strands of DNA creates a ‘Y’ shape called a replication ‘fork’. The two separated strands will act as templates for making the new strands of DNA.

Detailed explanation-3: -DNA denaturation is a process of separating dsDNA into single strands, which are favorable to DNA hybridization. Even though the denaturation is a key reaction that determines the success of DNA hybridization based bioassays, no systematic characterization of denaturation method for dsDNA has been attempted thus far.

Detailed explanation-4: -During DNA replication, DNA helicases unwind DNA at positions called origins where synthesis will be initiated. DNA helicase continues to unwind the DNA forming a structure called the replication fork, which is named for the forked appearance of the two strands of DNA as they are unzipped apart.

Detailed explanation-5: -What is Denaturation? DNA has a double-stranded helical structure. There are various factors that affect the stability of the DNA structure. In the denaturation process, the hydrogen bonds between two strands are broken giving rise to two single strands.