BIOMOLECULES AND ENZYMES

Detailed explanation-1: -The problem is solved with the help of an enzyme called primase. Primase makes an RNA primer, or short stretch of nucleic acid complementary to the template, that provides a 3’ end for DNA polymerase to work on.

Detailed explanation-2: -Elongation. During elongation, an enzyme called DNA polymerase adds DNA nucleotides to the 3′ end of the newly synthesized polynucleotide strand. The template strand specifies which of the four DNA nucleotides (A, T, C, or G) is added at each position along the new chain.

Detailed explanation-3: -Primase synthesizes RNA primers complementary to the DNA strand. DNA polymerase starts adding nucleotides to the 3’-OH end of the primer.

Detailed explanation-4: -For example, DNA polymerase III does most of the elongation work, adding nucleotides one by one to the 3’ end of the new and growing single strand.

Detailed explanation-5: -The DNA polymerase III holoenzyme is a complex, multisubunit enzyme that is responsible for the synthesis of most of the Escherichia coli chromosome.