BIOMOLECULES AND ENZYMES

Detailed explanation-1: -The enzyme performs best at pH 4–6 and 55–65 °C. Pullulanase secreted from A. oryzae and A.

Detailed explanation-2: -To date, five groups of pullulanase enzymes have been reported, that is, (i) pullulanase type I, (ii) amylopullulanase, (iii) neopullulanase, (iv) isopullulanase, and (v) pullulan hydrolase type III.

Detailed explanation-3: -The major difference between pullulanase and isoamylase is that pullulanases hydrolyse the -1, 6 glucosidic bond in pullulan and amylopectin, while isoamylase can only hydrolyse the -1, 6 bond in amylopectin and glycogen [12].