BIOMOLECULES AND ENZYMES

Detailed explanation-1: -Once the denaturing agent is removed, the original interactions between amino acids return the protein to its original conformation and it can resume its function. However, denaturation can be irreversible in extreme situations, like frying an egg.

Detailed explanation-2: -Protein denaturation is said to be irreversible when the denatured state achieved by increasing temperature or by using chemical denaturants is unable to return to the native, biologically functional state upon removal of the factor that caused denaturation.

Detailed explanation-3: -Denaturation can be brought about in various ways. Proteins are denatured by treatment with alkaline or acid, oxidizing or reducing agents, and certain organic solvents.

Detailed explanation-4: -The denaturation of many proteins, such as egg white, is irreversible. A common consequence of denaturation is loss of biological activity (e.g., loss of the catalytic ability of an enzyme).