BIOMOLECULES AND ENZYMES

Detailed explanation-1: -What is denaturing and how does it happen? A protein becomes denatured when its normal shape gets deformed because some of the hydrogen bonds are broken. Weak hydrogen bonds break when too much heat is applied or when they are exposed to an acid (like citric acid from lemon juice).

Detailed explanation-2: -A wide variety of reagents and conditions, such as heat, organic compounds, pH changes, and heavy metal ions can cause protein denaturation.

Detailed explanation-3: -The spectral changes show that the denaturation causes changes in the secondary structure, conformation of tyrosine residues, and conformation of disulfide bridges.

Detailed explanation-4: -Process of Denaturation of Proteins Heating, exposure to acids or bases and even violent physical action can cause denaturation to occur.