BIOMOLECULES AND ENZYMES

Detailed explanation-1: -The tryptophan indole ring reacts with glyoxylic acid in the presence of sulphuric acid (VI) to form a product of a red-violet colour.

Detailed explanation-2: -Interpretation The tryptophan indole ring reacts with glyoxylic acid in the presence of H2SO4 to form a product of a purple/violet colour. Glyoxylic acid is a polluting component of concentrated acetic acid. If a purple/violet ring appears between the two layers it confirms the presence of tryptophan in protein.

Detailed explanation-3: -Tryptophan reacts with glyoxylic acid in the presence of sulfuric acid and ferric chloride, producing a colored compound that absorbs at 560 nm.

Detailed explanation-4: -The Adamkiewicz reaction is part of a biochemical test used to detect the presence of the amino acid tryptophan in proteins.

Detailed explanation-5: -The Hopkins-Cole reaction, also known as the glyoxylic acid reaction, is a chemical test used for detecting the presence of tryptophan in proteins. A protein solution is mixed with Hopkins Cole reagent, which consists of glyoxylic acid. Concentrated sulfuric acid is slowly added to form two layers.