BIOMOLECULES AND ENZYMES

Detailed explanation-1: -The biuret test is a chemical test that can be used to check for the presence of peptide bonds in a given analyte. Therefore, the biuret test can also be used to gauge the amount of protein present in the analyte.

Detailed explanation-2: -Protein Detection (Activity) Proteins can be detected through the use of the Biuret test. Specifically, peptide bonds (C-N bonds) in proteins complex with Cu2+ in Biuret reagent and produce a violet color.

Detailed explanation-3: -The biuret method Copper salts in alkaline solution form a purple complex with substances containing two or more peptide bonds. The absorbance produced is proportional to the number of peptide bonds that are reacting and therefore to the number of protein molecules present in the reaction system.

Detailed explanation-4: -Peptide Bond Structure A peptide bond is a planar, trans and rigid configuration. It also shows a partial double bond character. The coplanarity of the peptide bond denotes the resonance or partial sharing of two pairs of electrons between the amide nitrogen and carboxyl oxygen.