BIOMOLECULES AND ENZYMES

BIOLOGY

PROTEINS

Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
Which are the denaturising factors?
A
Elevated temperature, organic solvents, acids, alkalis, heavy metal ions, concentrated solutions of urea or guanidine hydrochloride
B
Elevated temperature, acids, alkalis, heavy metal ions, concentrated solutions of urea or guanidine hydrochloride
C
Elevated temperature, organic solvents, alkalis, heavy metal ions, concentrated solutions of urea or guanidine hydrochloride
D
Elevated temperature, organic solvents, acids, heavy metal ions, concentrated solutions of urea or guanidine hydrochloride
Explanation: 

Detailed explanation-1: -Changes in pH, Increased Temperature, Exposure to UV light/radiation (dissociation of H bonds), Protonation amino acid residues, High salt concentrations are the main factors that cause a protein to denature.

Detailed explanation-2: -A wide variety of reagents and conditions, such as heat, organic compounds, pH changes, and heavy metal ions can cause protein denaturation.

Detailed explanation-3: -Elevated temperatures, extremes in pH, and changes in chemical or physical environment can all lead to protein denaturation. In general, things that destabilize H-bonding and other forces that contribute to secondary and tertiary protein structure will promote protein denaturation.

Detailed explanation-4: -The melting temperature varies for different proteins, but temperatures above 41°C (105.8°F) will break the interactions in many proteins and denature them. This temperature is not that much higher than normal body temperature (37°C or 98.6°F), so this fact demonstrates how dangerous a high fever can be.

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