BIOMOLECULES AND ENZYMES

BIOLOGY

PROTEINS

Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
Which colour form the amino acids that cointauins an alfa-amino group in a reaction with ninhydrine?
A
violet-red
B
violet-blue
C
red-blue
D
yellow-blue
Explanation: 

Detailed explanation-1: -The reaction of ninhydrin with an amino acid results in the creation of Ruhemann’s purple, a dark purple compound.

Detailed explanation-2: -Ninhydrin reacts with the amino acid in the fingerprint deposit (eccrine secretion) to give a dark-purple product. Amino acid-specific agents have particular application for the development of fingerprints on paper.

Detailed explanation-3: -Imino acids, e.g. proline and hydroxyproline, react with ninhydrin to give a yellow color.

Detailed explanation-4: -Ninhydrin is a white solid that is soluble in ethanol and acetone.

Detailed explanation-5: -The ‘alpha‘-amino acid which does not give purple colour in the ninhydrin test is. Solution : Ninhydrin test is highly specific for primary amines. Proline being a secondary amine gives a yellow orange colour with Nlnhydrin whereas all other ‘alpha‘-amino acids give a blue purple colour with Ninhydrin.

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