BIOMOLECULES AND ENZYMES

Detailed explanation-1: -A peptide has two ends: the end with a free amino group is called the N-terminal amino acid residue. The end with a free carboxyl group is called the C-terminal amino acid residue.

Detailed explanation-2: -The N-terminus (also known as the amino-terminus, NH2-terminus, N-terminal end or amine-terminus) is the start of a protein or polypeptide, referring to the free amine group (-NH2) located at the end of a polypeptide.

Detailed explanation-3: -The N-terminus denotes the beginning of a protein chain (first amino acid in the chain), and the C-terminus denotes the end of a protein chain (the last amino acid in the chain). The basic backbone of an amino acid is the nitrogen containing amino group, the central carbon, and the carboxylic acid group.

Detailed explanation-4: -Amino-terminal (N-terminal) sequence analysis is used to identify the order of amino acids of proteins or peptides, starting at their N-terminal end. This unit describes the sequence analysis of protein or peptide samples in solution or bound to PVDF membranes using a Perkin-Elmer Procise Sequencer.