CLINICAL MEDICINE

Detailed explanation-1: -WBC Counting area: The four large squares located at the corners of Neubauer’s Chamber are used for white blood cell count. RBC Counting area: The red blood cells are counted in the 5 squares of the Central square (divided into 25 squares, each of which is divided into 16 squares).

Detailed explanation-2: -Total leucocyte count Calculations: These are counted in the four large corner squares labeled as WBC and if the number is Y. One large area is 1 x 1 mm, and the depth is 0.1 mm. Total area counted in 4 large squares = 4 x 1 x o. 1 = 0.4 µL (4/10).

Detailed explanation-3: -Monolayer. This is usually a distance of one 10x field behind the feathered edge and is the optimal area for examination of cells, because it dries quickly and cells are well spread (not overlapping) and not disrupted. Red blood cells are separated or barely touching, with little overlapping.

Detailed explanation-4: -The hemacytometer chamber (Figure 1.2) is used to count both white and red blood cells. The large square areas indicated by the numbers 1, 2, 3, and 4 in the figure are used for counting white blood cells. The area indicated by the number 5 is used for counting red blood cells.