EVERYDAY SCIENCE

Detailed explanation-1: -A working curve is a plot of the analytical signal (the instrument or detector response) as a function of analyte concentratio.

Detailed explanation-2: -A calibration curve is a way to identify the concentration of an unknown substance. These curves use data points of known substances at varying concentrations, and researchers or developers can use these curves to find where an unknown substance plots.

Detailed explanation-3: -General use. In more general use, a calibration curve is a curve or table for a measuring instrument which measures some parameter indirectly, giving values for the desired quantity as a function of values of sensor output.

Detailed explanation-4: -How to calculate concentration from the calibration curve? Here you subtract the background b (the effect of the matrix) from the signal y, and then you divide by the sensitivity of the instrument used, a. The result is the concentration, x, with units depending on the technique with which the analysis is performed.