LIFE SCIENCE

OBJECTIVE LIFE SCIENCE

APPLIED BIOTECHNOLOGY

Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
Which substance creates DNA fragments with sticky or blunt ends to be joined with other DNA fragments?
A
plamid
B
DNA ligase
C
restriction enzyme
D
reverse transcriptase
Explanation: 

Detailed explanation-1: -Blunt-ended fragments can be joined to each other by DNA ligase.

Detailed explanation-2: -Answer and Explanation: Technically, restriction enzymes create DNA fragments with sticky ends.

Detailed explanation-3: -Blunt ends are also called non-cohesive ends, since there is no unpaired DNA strand fleeting at the end of DNA. The sticky ends, a.k.a. cohesive ends, have unpaired DNA nucleotides on either 5’-or 3’-strand, which are known as overhangs.

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