OBJECTIVE LIFE SCIENCE
BIOCHEMISTRY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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the enzyme catalase was denatured
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the temperature sped up the reaction too much to see the oxygen
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The enzyme was used up which prevent any further reactions.
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no oxygen in hydrogen peroxide to release
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Detailed explanation-1: -This is because the heat degraded the catalase enzyme, making it incapable of processing the hydrogen peroxide.
Detailed explanation-2: -A yeast sphere is dropped into a graduated cylinder containing H2O2, the substrate. The sphere will sink to the bottom of the cylinder and then, as the catalase produced by the yeast reacts with the hydrogen peroxide to form O2 gas bubbles around the sphere, the sphere will rise to the surface.
Detailed explanation-3: -[2] Catalase as an enzyme that belongs to the group of peroxydases is widely applied because of its ability for catalytic decomposition of hydrogen peroxide to water and oxygen. [3–5] Saccharomyces cerevisiae yeast is an abundant source of catalase.
Detailed explanation-4: -Mutations in the CAT gene greatly reduce the activity of catalase. A shortage of this enzyme can allow hydrogen peroxide to build up to toxic levels in certain cells. For example, hydrogen peroxide produced by bacteria in the mouth may accumulate in and damage soft tissues, leading to mouth ulcers and gangrene.
Detailed explanation-5: -Catalase works differently at different temperatures. Catalase activity increases as the temperature goes from 0’C to around 30’C. Catalase activity decreases as temperature goes from around 30’C to 100’C.