LIFE SCIENCE

OBJECTIVE LIFE SCIENCE

BIOCHEMISTRY

Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
The first step in the test for a lipid is to add ethanol or propanone. What does this step do?
A
dissolve the lipid
B
hydrolyse the lipid
C
emulsify the lipid
D
esterify the lipid
Explanation: 

Detailed explanation-1: -The emulsion test is a method to determine the presence of lipids using wet chemistry. The procedure is for the sample to be suspended in ethanol, allowing lipids present to dissolve (lipids are soluble in alcohols). The liquid (alcohol with dissolved fat) is then decanted into water.

Detailed explanation-2: -1 Add a few drops of ethanol to the food solution. 2 Shake the test tube and leave for one minute. 3 Pour the ethanol into a test tube of water. 4 If the solution turns cloudy, the food contains lipids.

Detailed explanation-3: -Sudan III is used to identify the presence of lipids in liquids. It will stain fat cells red.

Detailed explanation-4: -The Sudan IV test will test positive for lipids. The test procedure involves adding a few drops of Sudan IV to the test solution. Sudan IV is a dye that will stain lipids. If no lipids are present then the dye will sink to the bottom of the test tube.

There is 1 question to complete.