LIFE SCIENCE

Detailed explanation-1: -The correct answer: The factor which does NOT affect enzyme activity is d. Free Energy. The enzyme’s activity varies with the changing physical conditions.

Detailed explanation-2: -Enzyme activity is influenced by several environmental factors such as temperature, salt, pH, and substrate concentration. Low or high values of these factors will cause alteration in a structure that will further disrupt its functioning.

Detailed explanation-3: -Enzyme concentration does not affect the enzyme activity directly as substrate concentration determine it until the presence of the substrate in an excess amount. Enzymes are the assemblage of protein subunits and hence function under specific temperature and pH range only.

Detailed explanation-4: -The temperature and pH of the environment, as well as the concentration of the substrate and enzyme, all affect the rate at which an enzyme catalyzes a reaction. As a result, enzymes have optimal conditions in which they can work at peak efficiency.

Detailed explanation-5: -That’s because enzymes don’t affect the free energy of the reactants or products. Instead, enzymes lower the energy of the transition state, an unstable state that products must pass through in order to become reactants.