OBJECTIVE LIFE SCIENCE
BIOTECHNOLOGY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Which step of bacterial transformation occurs after a gene of interest has been isolated?
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removing the plasmid from the bacterium
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inserting the plasmid into a bacterium
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inserting the gene of the parent generation into the plasmid
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cutting the plasmid with restriction enzymes
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Explanation:
Detailed explanation-1: -In a typical cloning experiment, researchers first insert a piece of DNA, such as a gene, into a circular piece of DNA called a plasmid. This step uses restriction enzymes and DNA ligase and is called a ligation. After a ligation, the next step is to transfer the DNA into bacteria in a process called transformation.
Detailed explanation-2: -The Cloning Process. The DNA cloning process involves isolating the gene of interest and cutting it with restriction enzymes. The same enzymes are used to cut a vector, such as a bacterial plasmid. A bacterial plasmid is a small circular piece of DNA.
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