OBJECTIVE LIFE SCIENCE
MOLECULAR BIOLOGY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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T6-In order to insert a human gene into plasmid, both must
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code for the same gene product.
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be cut by the same restriction enzyme.
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originate from the same type of cell.
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have identical sequences.
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Explanation:
Detailed explanation-1: -When foreign DNA and plasmid are both cut with the same restriction enzyme and mixed together, all molecules will form recombinants.
Detailed explanation-2: -Using two different restriction enzyme sites can help ensure the correct orientation of the gene of interest when it is inserted and prevent the plasmid vector from ligating with itself.
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