OBJECTIVE LIFE SCIENCE
MOLECULAR BIOLOGY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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What happens in the Denature step of PCR?
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The DNA nucleotides are broken apart.
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The base-pairing rules for DNA are reversed.
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The double-stranded DNA is separated into two single strands of DNA.
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None of the above
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Explanation:
Detailed explanation-1: -During the first step in PCR, the starting solution is heated to the necessary temperature, usually between 90° and 100°C. As the heat builds, it breaks the bonds joining the two strands of the DNA double helix, thereby enabling the DNA to separate into two single strands.
Detailed explanation-2: -The heat breaks the hydrogen bonds of the original DNA sample and separates the DNA into single strands (this is termed denaturation of double-stranded DNA).
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