LIFE SCIENCE

OBJECTIVE LIFE SCIENCE

MOLECULAR BIOLOGY

Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
What happens in the Denature step of PCR?
A
The DNA nucleotides are broken apart.
B
The base-pairing rules for DNA are reversed.
C
The double-stranded DNA is separated into two single strands of DNA.
D
None of the above
Explanation: 

Detailed explanation-1: -During the first step in PCR, the starting solution is heated to the necessary temperature, usually between 90° and 100°C. As the heat builds, it breaks the bonds joining the two strands of the DNA double helix, thereby enabling the DNA to separate into two single strands.

Detailed explanation-2: -The heat breaks the hydrogen bonds of the original DNA sample and separates the DNA into single strands (this is termed denaturation of double-stranded DNA).

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