OBJECTIVE LIFE SCIENCE
MOLECULAR BIOLOGY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Which of the following is an enzymatic protein that acts as DNA glue?
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plasmid
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restriction enzyme
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ligase
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protein expression
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Explanation:
Detailed explanation-1: -Ligase, an enzyme that uses ATP to form bonds, is used in recombinant DNA cloning to join restriction endonuclease fragments that have annealed. The ligase commonly used is T4 DNA ligase, which was first isolated from E. coli that were infected with the lytic bacteriophage T4.
Detailed explanation-2: -Ligase is an enzyme that catalyzes the binding of two molecules. An example is a DNA ligase that links two fragments of DNA by forming a phosphodiester bond.
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