BIOTECHNOLOGY
APPLICATIONS OF BIOTECHNOLOGY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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ori
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selectable marker
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both of above
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non of these
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Detailed explanation-1: -A cloning vector generally refers to a small DNA fragment into which a foreign DNA of interest can be inserted. The vector DNA should be of a small size. It must be self-replicating inside the host cell. The vector DNA must have an ori(locus) and restriction site.
Detailed explanation-2: -A cloning vector should possess an origin of replication so that it can self-replicate inside the host cell. It should have a restriction site for the insertion of the target DNA. It should have a selectable marker with an antibiotic resistance gene that facilitates screening of the recombinant organism.
Detailed explanation-3: -A DNA molecule in which when a foreign DNA is integrated has the capability to replicate it within itself to give birth to numerous clones of recombinant DNA is known as a cloning vector. Vectors that are used for cloning are plasmids and phages.
Detailed explanation-4: -Characteristics that engineered vectors are made to possess are: Easy linking of foreign DNA. Selection of recombinants from non-recombinants/selectable markers.