BIOTECHNOLOGY
APPLICATIONS OF BIOTECHNOLOGY
Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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.Peptidase
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.Amylases
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.Lipases
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.Nucleases
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Detailed explanation-1: -Restriction enzymes belong to a larger class of enzymes called Nucleases. They cut DNA at special pelindromic sequence. Restriction enzymes are of two types-Restriction endonuclease and Restriction exonuclease.
Detailed explanation-2: -Restriction enzymes are one class of the broader endonuclease group of enzymes.
Detailed explanation-3: -Exonucleases and endonucleases are two classes of restriction enzymes. The function of Exonucleases is to remove the nucleotides from the ends of the DNA while the Endonucleases play a major role in cutting the DNA at specific sites between the ends of DNA.
Detailed explanation-4: -restriction enzyme, also called restriction endonuclease, a protein produced by bacteria that cleaves DNA at specific sites along the molecule. In the bacterial cell, restriction enzymes cleave foreign DNA, thus eliminating infecting organisms.
Detailed explanation-5: -Restriction enzymes are traditionally classified into four types on the basis of subunit composition, cleavage position, sequence specificity and cofactor requirements.