A eukaryotic gene has sticky ends produced by the restriction endonuclease EcoRI. The gene is added to a mixture containing EcoRI and a bacterial plasmid that carries two genes conferring resistance to ampicillin and tetracycline. The plasmid has one recognition site for EcoRI located in the tetracycline resistance gene. This mixture is incubated for several hours, exposed to DNA ligase, and then added to bacteria growing in nutrient broth. The bacteria are allowed to grow overnight and are streaked on a plate using a technique that produces isolated colonies that are clones of the original. Samples of these colonies are then grown in four different media:nutrient broth plus ampicillin, nutrient broth plus tetracycline, nutrient broth plus ampicillin and tetracycline, and nutrient broth without antibiotics.Bacteria containing a plasmid into which the eukaryotic gene has integrated would grow in .....

MOLECULAR BASIS OF INHERITANCE

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Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]

A eukaryotic gene has “sticky ends” produced by the restriction endonuclease EcoRI. The gene is added to a mixture containing EcoRI and a bacterial plasmid that carries two genes conferring resistance to ampicillin and tetracycline. The plasmid has one recognition site for EcoRI located in the tetracycline resistance gene. This mixture is incubated for several hours, exposed to DNA ligase, and then added to bacteria growing in nutrient broth. The bacteria are allowed to grow overnight and are streaked on a plate using a technique that produces isolated colonies that are clones of the original. Samples of these colonies are then grown in four different media:nutrient broth plus ampicillin, nutrient broth plus tetracycline, nutrient broth plus ampicillin and tetracycline, and nutrient broth without antibiotics.Bacteria containing a plasmid into which the eukaryotic gene has integrated would grow in ____

A	the nutrient broth only.
B	the ampicillin broth and the nutrient broth.
C	all four types of broth.
D	the nutrient broth and the tetracycline broth only.

Explanation:

Detailed explanation-1: -A eukaryotic gene has “sticky ends” produced by the restriction endonuclease EcoRI. The gene is added to a mixture containing EcoRI and a bacterial plasmid that carries two genes conferring resistance to ampicillin and tetracycline.

Detailed explanation-2: -These are named so because they form hydrogen bonds with their complementary cut counterparts. This stickiness of the ends facilitates the action of the enzyme DNA ligase during ligation with desired DNA.

Detailed explanation-3: -EcoRI is a restriction enzyme that is derived from the bacteria E. coli. This restriction enzyme produces sticky ends when it cuts DNA at the restriction site. EcoRI recognizes a specific sequence on DNA: GAATTC.

Detailed explanation-4: -Which of the following techniques used to analyze gene function depends on the specificity of DNA base complementarity? Silencing of selected genes is often done using RNA interference (RNAi).

There is 1 question to complete.

NEET BIOLOGY

GENETICS AND EVOLUTION

MOLECULAR BASIS OF INHERITANCE