NEET BIOLOGY

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GENETICS AND EVOLUTION

MOLECULAR BASIS OF INHERITANCE

Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
A human gene is cloned in E. coli and then allowed to undergo gene expression. Upon performing downstreaming processes and testing for functionality of that gene product, it was found to be inactive. Which of the following can be a promisable reason for same?
A
Coupled Transcription and Translation
B
Single type of RNA polymerase
C
Lack of Post-trancriptional machinery
D
Presence of extrachromosomal DNA along with genomic DNA
Explanation: 

Detailed explanation-1: -Recombinant DNA is a technology scientists developed that made it possible to insert a human gene into the genetic material of a common bacterium. This “recombinant” micro-organism could now produce the protein encoded by the human gene.

Detailed explanation-2: -E. coli is a preferred host for gene cloning due to the high efficiency of introduction of DNA molecules into cells. E. coli is a preferred host for protein production due to its rapid growth and the ability to express proteins at very high levels.

Detailed explanation-3: -recombinant DNA, molecules of DNA from two different species that are inserted into a host organism to produce new genetic combinations that are of value to science, medicine, agriculture, and industry.

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