GENETICS AND EVOLUTION
MOLECULAR BASIS OF INHERITANCE
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Strand-binding Proteins
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Transportation Proteins
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Leading Strand Proteins
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Regular Proteins
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Detailed explanation-1: -During DNA replication, the single-stranded DNA binding protein (SSB) wraps single-stranded DNA (ssDNA) with high affinity to protect it from degradation and prevent secondary structure formation.
Detailed explanation-2: -In E. coli, this protein is a tetramer known as single-stranded-binding (SSB) protein, which the ssDNA winds itself around, while in T4 and T7, these proteins are monomers known as gp32 and gp2. 5, respectively.
Detailed explanation-3: -SSBs (and RPAs) stabilize the separated strands through rapid binding that ensures complete coating of the ssDNA as it emerges from the helicase.
Detailed explanation-4: -As single-stranded DNA binding proteins are utilized in some of the most important aspects of DNA metabolism, they are used extensively in DNA replication, repair and recombination.
Detailed explanation-5: -SSBs are essential proteins found in all domains of life. SSBs bind ssDNA with high affinity and in a sequence-independent manner and, in doing so, SSBs help to form the central nucleoprotein complex substrate for DNA replication, recombination, and repair processes.