SSC
BIOLOGY
Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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What happens during the formation of Okazaki fragments?
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DNA polymerase I adds nucleotides in the 5´
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RNA polymerase adds nucleotides in the 3´
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DNA polymerase III adds nucleotides in the 5´
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DNA polymerase III adds nucleotides in the 3´
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Explanation:
Detailed explanation-1: -Primase adds RNA primers onto the lagging strand, which allows synthesis of Okazaki fragments from 5’ to 3’. However, primase creates RNA primers at a much lower rate than that at which DNA polymerase synthesizes DNA on the leading strand.
Detailed explanation-2: -DNA polymerase is able to add nucleotides only in the 5’ to 3’ direction (a new DNA strand can be only extended in this direction). It also requires a free 3’-OH group to which it can add nucleotides by forming a phosphodiester bond between the 3’-OH end and the 5’ phosphate of the next nucleotide.
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