BIOMOLECULES AND ENZYMES

Detailed explanation-1: -Size-exclusion chromatography (SEC), also known as molecular sieve chromatography, is a chromatographic method in which molecules in solution are separated by their size, and in some cases molecular weight. It is usually applied to large molecules or macromolecular complexes such as proteins and industrial polymers.

Detailed explanation-2: -Western Blotting The most common version of Western blotting is known as immunoblotting. In this technique a sample of proteins is first electrophoresed by SDS-PAGE to separate the proteins on the basis of their molecular weights.

Detailed explanation-3: -Gel filtration chromatography is a well-accepted method for determining the size and molecular weight of proteins.

Detailed explanation-4: -Size exclusion chromatography (SEC), also called gel filtration chromatography, separates molecules based on their sizes. SEC resins are gels that contain beads with a known pore size.

Detailed explanation-5: -Larger molecules take longer to move up the chromatography paper or TLC plate, whereas smaller molecules are more mobile. Likewise, the polarity of the molecules can affect how far the spots travel, depending on the type of solvent used.