BIOMOLECULES AND ENZYMES

Detailed explanation-1: -If no colour change is observed, the analyte does not contain amino acids, amines, or ammonia.

Detailed explanation-2: -Result and Interpretation of Ninhydrin Test This colouration denotes the presence of an amino acid in the sample. The absence of the complex in the test tube constitutes a negative result and demonstrates that the sample does not contain any amino acids.

Detailed explanation-3: -Ninhydrin Test Positive result: A purple-colored complex found in the tube is a positive result. This indicates that there are amino acids in the sample. Ninhydrin Test Negative Result :A negative result is when the tube does not contain the complex. This indicates that there are no amino acids in the sample.

Detailed explanation-4: -Only proteins give positive ninhydrin test. They give blue colour with ninhydrin.

Detailed explanation-5: -Three drops of 1% solution of ninhydrin in ethanol is added to 1 ml of the solution and the solution heated for five minutes in a boiling water bath. A positive test is indicated by: the formation of red, blue or purple color.