BIOMOLECULES AND ENZYMES

BIOLOGY

AMINO ACIDS

Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
In chromatography, it is usually a high-quality filter paper or an adsorbent.
A
mobile phase
B
stationary phase
C
blank phase
D
None of the above
Explanation: 

Detailed explanation-1: -The filter paper acts as the stationary phase while the solvent acts as the mobile phase.

Detailed explanation-2: -The Whatman filter paper, which is made up of 99% −cellulose, binds the water tightly to the paper and hence it is called as stationary phase.

Detailed explanation-3: -For adsorption chromatography, the adsorbent is used as the stationary phase. The solute binds to the adsorbent via van der Waal forces and steric interactions. Since the adsorption sites are typical only on the outer surface of the stationary phase, fairly small particles are used as the stationary phase.

Detailed explanation-4: -Chromatography is a technique for separating a sample into its component parts. Paper chromatography is a primitive form of chromatography where filter paper acts as a stationary phase & solvent acts as mobile phase.

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