BIOMOLECULES AND ENZYMES

Detailed explanation-1: -The reaction of ninhydrin with an amino acid results in the creation of Ruhemann’s purple, a dark purple compound.

Detailed explanation-2: -The reaction between ninhydrin and an amino acid, leading to the production of a dark purple compound known as Ruhemann’s purple.

Detailed explanation-3: -The ninhydrin test is a chemical test useful to identify ammonia, primary/secondary amines, or amino acids. In this test, ninhydrin reagent is added to the test material, resulting in the production of deep blue colour, also known as Ruhemann’s purple, in the presence of an amino group.

Detailed explanation-4: -Ruhemann’s purple is formed from nitrite, ascorbate, and ninhydrin with the formation of variable amounts of ammonia. The reaction begins with the nitrosation of ninhydrin to form nitrosoninhydrin, which will release nitric oxide.

Detailed explanation-5: -Ninhydrin (2, 2-dihydroxyindane-1, 3-dione) is an organic compound with the formula C6H4(CO)2C(OH)2. It is used to detect ammonia and amines. Upon reaction with these amines, ninhydrin gets converted into deep blue or purple derivatives, which are called Ruhemann’s purple.