BIOMOLECULES AND ENZYMES

BIOLOGY

AMINO ACIDS

Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
It is a test used to detect presence of guanidino group in an amino acid.
A
Sakaguchi Test
B
Xanthoproteic Test
C
Lead Acetate Test
D
Hopkins-Cole Test
Explanation: 

Detailed explanation-1: -The Sakaguchi reagent is used to test for a certain amino acid and proteins. The amino acid that is detected in this test is arginine. Since arginine has a guanidine group in its side chain, it gives a red color with -naphthol in the presence of an oxidizing agent like bromine solution.

Detailed explanation-2: -The Sakaguchi test is a chemical test used for detecting the presence of arginine in proteins. It is named after the Japanese Food Scientist and Organic Chemist, Shoyo Sakaguchi (1900–1995) who described the test in 1925. Principle: Sakaguchi test is given by guanidinium compounds.

Detailed explanation-3: -The Sakaguchi reaction is a colorimetric reaction for identification and quantitation of guanidino groups, involving reaction with naphthol and sodium hypochlorite, providing a qualitative test for arginine that is free or in protein (10). This reaction was used by Fogg to show that heterocysts contain arginine (11).

Detailed explanation-4: -The Sakaguchi test is a chemical test used to detect presence of arginine in proteins. It is named after the Japanese food scientist and organic chemist, Shoyo Sakaguchi (1900–1995) who described the test in 1925.

Detailed explanation-5: -Sakaguchi test is an example of color reactions or test that is performed for the detection of amino acids or proteins. The test is a specific test for arginine where the guanidinium group of arginine reacts with 1-naphthol or -naphthol to produce a colored product.

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