BIOMOLECULES AND ENZYMES

Detailed explanation-1: -The biuret test is a chemical test that can be used to check for the presence of peptide bonds in a given analyte. Therefore, the biuret test can also be used to gauge the amount of protein present in the analyte.

Detailed explanation-2: -In chemistry, the Biuret test (IPA: /ˌbaɪjəˈrɛt/, /ˈbaɪjəˌrɛt/), also known as Piotrowski’s test, is a chemical test used for detecting the presence of at least two peptide bonds in a molecule.

Detailed explanation-3: -Significance of Biuret Test It is widely used in determining the protein amount in urine. Biuret test for protein is also useful to total protein’s quantitative determination, using spectrophotometric analysis.

Detailed explanation-4: -The presence of protein is tested by the Biuret test for proteins. The Biurette reagent made of sodium hydroxide and copper (II) sulphate helps in determining the presence of protein in a sample.

Detailed explanation-5: -The biuret method Thus, the biuret reaction with proteins is suitable for the determination of total protein by spectrophotometry (at 540–560 nm). The method is used extensively in clinical laboratories, particularly in automated analyzers in which protein concentration can be measured down to 0.1–0.15 g l−1.