BIOMOLECULES AND ENZYMES

Detailed explanation-1: -Xanthoproteic test is used to detect amino acids containing an aromatic nucleus (tyrosine, tryptophan and phenylalanine) in a protein solution which gives yellow color nitro derivatives on heating with conc. HNO3. The aromatic benzene ring undergoes nitration to give yellow colored product.

Detailed explanation-2: -The Xanthoproteic test uses a nitration reaction to determine the presence of proteins in a solution. When the sample is treated with a hot, concentrated nitric acid it reacts with aromatic amino acids such as phenylalanine, tyrosine and tryptophan and forms a yellow colored product known as Xantho protein.

Detailed explanation-3: -Xanthoproteic test is specific for protein containing aromatic amino acids. The benzene ring in the amino acids is nitrated by heating with nitric acid and forms yellow nitro-compounds which turns to orange colour with alkali.

Detailed explanation-4: -Xanthoproteic test is used to detect the presence of aryl amino acids.

Detailed explanation-5: -Xanthoproteic Test When nitric acid is added to a sample and the mixture is heated, a yellow solution will result if the sample contains tyrosine or tryptophan.