BIOMOLECULES AND ENZYMES

Detailed explanation-1: -Sakaguchi test is a biochemical test consisting of colorimetric reaction for the detection and quantification of guanidinium groups, used as a qualitative test for arginine that is either free or in protein. The test was discovered by and named after the Japanese Food Scientist Shoyo Sakaguchi in 1925.

Detailed explanation-2: -f) Sakaguchi Test: The Sakaguchi reagent is used to test for a certain amino acid and proteins. The amino acid that is detected in this test is arginine. Since arginine has a guanidine group in its side chain, it gives a red color with -naphthol in the presence of an oxidizing agent like bromine solution.

Detailed explanation-3: -The Sakaguchi reaction is a colorimetric reaction for identification and quantitation of guanidino groups, involving reaction with naphthol and sodium hypochlorite, providing a qualitative test for arginine that is free or in protein (10). This reaction was used by Fogg to show that heterocysts contain arginine (11).

Detailed explanation-4: -The Sakaguchi test is a chemical test used to detect presence of arginine in proteins. It is named after the Japanese food scientist and organic chemist, Shoyo Sakaguchi (1900–1995) who described the test in 1925.

Detailed explanation-5: -Sakaguchi test is Specific for Arginine Sakaguchi’s test is positive for the amino acid containing the guanidine group in Arginine. Guanidine group present in the amino acid reacts with -Naphthol and alkaline hypobromite to give red-coloured complex.