BIOMOLECULES AND ENZYMES

Detailed explanation-1: -At the isoelectric point, the amino acid has a net zero charge due to having a positive charge on its amino functional group and a negative charge on its carboxyl functional group. This form is referred to as the zwitterion and occurs at a specific pH for each amino acid.

Detailed explanation-2: -1). When an amino acid contains both a plus and a minus charge in the “backbone", it is called a zwitterion and has an overall neutral charge. The zwitterion of an amino acid exists at a pH equal to the isoelectric point. Each amino acid has its own pI value based on the properties of the amino acid.

Detailed explanation-3: -At a certain pH of the medium called an isoelectric point of an -amino acid, the Zwitterion behaves as neutral and does not migrate to any electrode on passing current. The isoelectric points are in the pH range of 5. 5 to 6.

Detailed explanation-4: -L-lysine zwitterion is a L-alpha-amino acid zwitterion resulting from the carboxy proton transfer to the L-lysine amino group; the dominant species at pH 7.3.

Detailed explanation-5: -The isoelectric point is the pH at which a zwitterion is uncharged. The pH can affect the charge of a molecule by introducing protons (H+). The amino group of an amino acid is a very effective proton acceptor, so is considered to be basic. The carboxyl group is an effective proton donor and is considered to be acidic.