BIOMOLECULES AND ENZYMES

Detailed explanation-1: -All carbohydrates (monosaccharides, disaccharides, and polysaccharides) pass the Molisch test. It is based on Sulphuric acid dehydrating the carbohydrate to produce an aldehyde, which condenses with two molecules of -naphthol, resulting in the appearance of a purple ring at the interface.

Detailed explanation-2: -A purple ring appears at the interface between the acid and test layers which confirms the presence of carbohydrates. The presence of carbohydrates is indicated if the test solution creates purple or purplish-red tinted layers. As a result, the Molisch test results are positive.

Detailed explanation-3: -The purple ring of Molisch reaction is due to Furfural + Napthol.

Detailed explanation-4: -A positive reaction is indicated by appearance of a purple red ring at the interface between the acid and test layers.

Detailed explanation-5: -(a) Molisch’s Test: In this test, concentrated sulfuric acid converts the given carbohydrate into furfural or its derivatives, which react with -naphthol to form a purple coloured product.