BIOMOLECULES AND ENZYMES

BIOLOGY

CLASSIFICATION OF CARBOHYDRATES

Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
As you add 1 mL Fructose + 2 mL Benedict’s reagent, what was the final color of the solution?
A
green/yellow
B
blue
C
orange/red
D
dark blue
Explanation: 

Detailed explanation-1: -Add 2 mL of Benedict’s reagent to it and boil. Direct heating of test tube should not be done. It should be carried out in a water bath at the boiling point of water. The colour changes from blue to green and finally to orange or brick red indicating the presence of simple sugar (glucose).

Detailed explanation-2: -Fructose is heated with Benedict’s reagent to form a red precipitate.

Detailed explanation-3: -We can use a special reagent called Benedict’s solution to test for simple carbohydrates like glucose. Benedict’s solution is blue but, if simple carbohydrates are present, it will change colour – green/yellow if the amount is low and red if it is high.

Detailed explanation-4: -Benedict’s reagent starts out aqua-blue. As it is heated in the presence of reducing sugars, it turns yellow to orange. The “hotter” the final color of the reagent, the higher the concentration of reducing sugar.

Detailed explanation-5: -Result Interpretation of Benedict’s Test If it changes to orange, then it means that 1 to 1.5 percent sugar is present. If color changes to red, then 1.5 to 2.0 percent sugar is present. And if color changes to brick red, it means that more than 2 percent sugar is present in solution.

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