BIOMOLECULES AND ENZYMES

Detailed explanation-1: -Over a period of time, enzymes will be deactivated at even moderate temperatures. Storage of enzymes at 5°C or below is generally the most suitable. Lower temperatures lead to slower chemical reactions.

Detailed explanation-2: -Incubation at 65°C for 20 minutes inactivates the majority of restriction endonucleases that have an optimal incubation temperature of 37°C. Enzymes that cannot be inactivated at 65°C can often be inactivated by incubation at 80°C for 20 minutes.

Detailed explanation-3: -Why did the enzyme activity differ at 0°C and at 100°C? The enzyme activity increases as the temperature rises due to the substrates colliding withthe enzymes’ active sites more frequently at higher temperatures.

Detailed explanation-4: -There is a certain temperature at which an enzyme’s catalytic activity is at its greatest (see graph). This optimal temperature is usually around human body temperature (37.5 oC) for the enzymes in human cells.

Detailed explanation-5: -Enzymes are also subject to cold denaturation, leading to the loss of enzyme activity at low temperatures [11]. This phenomenon is thought to occur through the hydration of polar and non-polar groups of proteins [12], a process thermodynamically favoured at low temperatures.