BIOMOLECULES AND ENZYMES

Detailed explanation-1: -Thiamin (Vitamin B1) Thiamin pyrophosphate (TPP) is a cofactor for a number of enzymes, such as transketolase, pyruvate dehydrogenase, and -ketoglutarate dehydrogenase.

Detailed explanation-2: -Thiamin (B1) is essential for the oxidative decarboxylation of the multienzyme branched-chain ketoacid dehydrogenase complexes of the citric acid cycle.

Detailed explanation-3: -Thiamine pyrophosphate (TPP) occurs in virtually all organisms as coenzyme for enzymes catalyzing the decarboxylation of 2-keto acids and the transfer of C2 units. The simplest method for TPP determination is its chemical oxidation to thiochrome pyrophosphate and measurement by fluorimetry.

Detailed explanation-4: -Oxidative Decarboxylation of -Ketoglutarate to Succinyl-CoA -Ketoglutarate is reversibly transaminated to glutamate, and it is utilized in the hydroxylation of prolyl or lysyl residues of collagen (Chapter 23).