HORTICULTURE

Detailed explanation-1: -High-resolution transmission electron microscopy (HRTEM) is an imaging mode of the TEM that allows the imaging of the crystallographic structure of a sample at an atomic scale. Due to its high resolution, it is a valuable tool to study nanoscale properties of crystalline material.

Detailed explanation-2: -At present, the highest point resolution realised in phase contrast transmission electron microscopy is around 0.5 ångströms (0.050 nm).

Detailed explanation-3: -TEM uses similar principles to conventional light microscopy, but electrons are transmitted through a sample to produce an image, rather than light. This technique is a very reliable way to produce high-resolution images, which rely on the interaction of electrons with the atoms in the sample.

Detailed explanation-4: -SEM vs TEM: Differences in operation TEM users can magnify their samples by more than 50 million times, while for the SEM, this is limited to 1–2 million times. However, the maximum field of view (FOV) that SEMs can achieve is far larger than TEMs, meaning TEM users can only image a very small part of their sample.

Detailed explanation-5: -Transmission electron microscopes are capable of imaging at a significantly higher resolution than light microscopes, owing to the smaller de Broglie wavelength of electrons.